Hotspot

trnS-GCU-trnG-UCC

LSC · 49421-50487 · 1067 bp

Back to Racinaea

Pi 0.0159

MarkerSeek score 63.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1067
variable_sites: 23
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	55.0	50.0	58.0	58.3	1017-1274 bp	1.0	82.7
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	962-1219 bp	1.0	82.7
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	55.0	50.0	58.0	57.5	1018-1275 bp	1.0	81.4
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	963-1220 bp	1.0	81.3
trnS-GCU-trnG-UCC_p5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	55.0	50.0	58.0	57.4	1016-1273 bp	1.0	81.0