Hotspot

trnV-GAC-rps12

IRb · 25131-26843 · 1713 bp

Back to Racinaea

Pi 0.00117
MarkerSeek score 59.10
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRb
length_bp
1713
variable_sites
3
pi
0.00117
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1870 bp 1.0 77.1
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1809 bp 1.0 76.8
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1813 bp 1.0 76.7
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1808 bp 1.0 76.7
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1811 bp 1.0 75.8