Hotspot

rpl32-trnL-UAG

SSC · 175-898 · 724 bp

Back to Racinaea

Pi 0.0113
MarkerSeek score 61.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
724
variable_sites
12
pi
0.0113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CACCGGGCATTCAAAAAGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.5 58.0 811-868 bp 1.0 78.6
rpl32-trnL-UAG_p2 CACCGGGCATTCAAAAAGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.5 58.0 812-869 bp 1.0 78.6
rpl32-trnL-UAG_p3 CACCGGGCATTCAAAAAGTT TTTAGAAGATGCCGCCACTC 45.0 50.0 57.5 58.0 871-928 bp 1.0 78.3
rpl32-trnL-UAG_p4 TTCCACCGGGCATTCAAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 814-871 bp 1.0 78.1
rpl32-trnL-UAG_p5 TTTCCACCGGGCATTCAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 815-872 bp 1.0 78.1