Hotspot

rpoC2

LSC · 18524-22702 · 4179 bp

Back to Quillaja

Pi 0.00287

MarkerSeek score 42.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4179
variable_sites: 12
pi: 0.00287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATTTCTTCGCCTCTTTGCCA	GCAGTCCCTTTTTCACTCCA	45.0	50.0	58.1	58.0	264 bp	1.0	79.7
rpoC2_p2	ATTTCTTCGCCTCTTTGCCA	TGCAGTCCCTTTTTCACTCC	45.0	50.0	58.1	58.0	265 bp	1.0	79.7
rpoC2_p3	TTCTTCGCCTCTTTGCCAAT	GCAGTCCCTTTTTCACTCCA	45.0	50.0	58.1	58.0	262 bp	1.0	79.7
rpoC2_p4	TTCTTCGCCTCTTTGCCAAT	TGCAGTCCCTTTTTCACTCC	45.0	50.0	58.1	58.0	263 bp	1.0	79.7
rpoC2_p5	CCCGCACATAGGATTCGATT	GCAGTCCCTTTTTCACTCCA	50.0	50.0	57.8	58.0	194 bp	1.0	79.3