Hotspot

clpP1

LSC · 74920-76884 · 1965 bp

Back to Quillaja

Pi 0.00611

MarkerSeek score 61.40

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1965
variable_sites: 12
pi: 0.00611
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	CGTGAATTTGTGCGGAATCC	ACGCTTCCACATCAAAGTGA	50.0	45.0	58.1	57.7	2042-2044 bp	1.0	77.7
clpP1_p2	CGTGAATTTGTGCGGAATCC	CCATCGTTACGCTTCCACAT	50.0	50.0	58.1	58.3	2050-2052 bp	1.0	76.9
clpP1_p3	TGAATTTGTGCGGAATCCCT	ACGCTTCCACATCAAAGTGA	45.0	45.0	57.8	57.7	2040-2042 bp	1.0	76.8
clpP1_p4	GTGAATTTGTGCGGAATCCC	ACGCTTCCACATCAAAGTGA	50.0	45.0	57.7	57.7	2041-2043 bp	1.0	76.3
clpP1_p5	CGTGAATTTGTGCGGAATCC	TCGTTACGCTTCCACATCAA	50.0	45.0	58.1	57.6	2047-2049 bp	1.0	76.2