Hotspot

rbcL-accD

LSC · 61028-61884 · 857 bp

Back to Quillaja

Pi 0.00585

MarkerSeek score 50.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 5
pi: 0.00585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	CAACATCGAATTGAACCACCA	50.0	42.9	58.0	57.1	962-981 bp	1.0	65.0
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	ACAACATCGAATTGAACCACC	50.0	42.9	58.0	57.1	963-982 bp	1.0	65.0
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	CAACATCGAATTGAACCACCA	55.0	42.9	58.0	57.1	977-996 bp	1.0	64.9
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	ACAACATCGAATTGAACCACC	55.0	42.9	58.0	57.1	978-997 bp	1.0	64.9
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	GACAACATCGAATTGAACCACC	50.0	45.5	58.0	58.2	964-983 bp	1.0	62.9