Hotspot

psaA-pafI

LSC · 45733-46529 · 797 bp

Back to Quillaja

Pi 0.00627

MarkerSeek score 62.40

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 5
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGAGAAATGACCGGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	994 bp	1.0	79.3
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	975 bp	1.0	79.2
psaA-pafI_p3	TGAGAAATGACCGGGTTTGG	AGCTATAGCCCTTACTCCCG	50.0	55.0	58.1	58.1	969 bp	1.0	79.1
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACTCCCG	50.0	55.0	57.9	58.1	950 bp	1.0	78.9
psaA-pafI_p5	TGAGAAATGACCGGGTTTGG	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	936 bp	1.0	78.8