Hotspot

psbZ-trnG-GCC

LSC · 39527-40334 · 808 bp

Back to Quillaja

Pi 0.0099

MarkerSeek score 55.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 8
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGGTACATCATTATGGATTGGA	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	920-951 bp	1.0	54.6
psbZ-trnG-GCC_p2	TCCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	921-952 bp	1.0	54.6
psbZ-trnG-GCC_p3	CCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	45.5	50.0	56.7	57.8	920-951 bp	1.0	52.9
psbZ-trnG-GCC_p4	TGGATTAGTCTTTCTAGTGGGT	CGTCTTCTCCTTGGCAAAGA	40.9	50.0	55.9	57.8	901-932 bp	1.0	46.6
psbZ-trnG-GCC_p5	CCGGTACATCATTATGGATTGGA	CGGATAGCGGGAATCGAAC	43.5	57.9	58.1	58.2	942-973 bp	1.0	46.4