Hotspot

trnE-UUC-trnT-GGU

LSC · 33802-34592 · 791 bp

Back to Quillaja

Pi 0.00382

MarkerSeek score 61.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 3
pi: 0.00382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CCATGATCAACTTCCCCTCG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	948-962 bp	1.0	80.4
trnE-UUC-trnT-GGU_p2	CCATGATCAACTTCCCCTCG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	927-941 bp	1.0	79.9
trnE-UUC-trnT-GGU_p3	CCATGATCAACTTCCCCTCG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	924-938 bp	1.0	79.8
trnE-UUC-trnT-GGU_p4	AACTTCCCCTCGTAGTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	940-954 bp	1.0	79.6
trnE-UUC-trnT-GGU_p5	CCATGATCAACTTCCCCTCG	CCGATGACTTACGCCTTACC	55.0	55.0	58.0	58.2	945-959 bp	1.0	79.2