Hotspot

trnK-UUU-rps16

LSC · 4623-5617 · 995 bp

Back to Quillaja

Pi 0.0062

MarkerSeek score 51.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 6
pi: 0.0062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCTGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1221-1235 bp	1.0	82.0
trnK-UUU-rps16_p2	CCTTTCTGGATCAGTCGTGG	ATTGCGAATAGGAGCGGTTT	55.0	45.0	58.0	58.0	1558-1572 bp	1.0	81.5
trnK-UUU-rps16_p3	TCTGGATCAGTCGTGGTCTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1217-1231 bp	1.0	81.5
trnK-UUU-rps16_p4	TTCTGGATCAGTCGTGGTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1218-1232 bp	1.0	81.5
trnK-UUU-rps16_p5	CCTTTCTGGATCAGTCGTGG	AAAAGGGAGTAGAGACCGCT	55.0	50.0	58.0	58.1	1645-1659 bp	1.0	81.2