Hotspot

trnH-GUG-psbA

LSC · 242-666 · 425 bp

Back to Quillaja

Pi 0.0118

MarkerSeek score 48.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	542-543 bp	1.0	79.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	556-557 bp	1.0	79.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	555-556 bp	1.0	79.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	557-558 bp	1.0	79.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TGCCTCTAGGAATCTCTGGG	55.0	55.0	58.1	57.9	995-996 bp	1.0	78.7