Hotspot

ycf1

SSC · 130633-135240 · 4608 bp

Back to Quercus

Pi 0.00779

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4608
variable_sites: 334
pi: 0.00779
unique_haplotype_count: 83
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTGGTATGACCCATGGT	TCTGACGATTCATCAAGATCAGA	45.0	39.1	56.2	57.2	182 bp	0.991304	47.7
ycf1_p2	TGATTGGTATGACCCATGGTT	TCTGACGATTCATCAAGATCAGA	42.9	39.1	57.0	57.2	182 bp	0.991304	47.7
ycf1_p3	TTGATTGGTATGACCCATGGT	TCTGACGATTCATCAAGATCAGA	42.9	39.1	57.0	57.2	183 bp	0.991304	47.7
ycf1_p4	TGATTGGTATGACCCATGGT	TGATAACGTTACCAACAGGA	45.0	40.0	56.2	54.0	272 bp	0.991304	46.7
ycf1_p5	TGATTGGTATGACCCATGGT	TGATAACGTTACCAACAGGAA	45.0	38.1	56.2	54.9	272 bp	0.991304	46.7