Hotspot

atpF

LSC · 13576-14909 · 1334 bp

Back to Quercus

Pi 0.00388

MarkerSeek score 58.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1334
variable_sites: 58
pi: 0.00388
unique_haplotype_count: 54
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACCCGCCATTACTTCATCA	GCCAAGCTGTAGAAGGGATC	50.0	55.0	58.0	58.0	2064-2167 bp	1.0	87.0
atpF_p2	CACCCGCCATTACTTCATCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2149-2252 bp	1.0	86.9
atpF_p3	CACCCGCCATTACTTCATCA	ACACTAACCCATGGGAAGGA	50.0	50.0	58.0	57.9	1826-1918 bp	0.991304	86.6
atpF_p4	CACCCGCCATTACTTCATCA	CCATGGGAAGGACTGATTGG	50.0	55.0	58.0	57.9	1818-1910 bp	0.991304	86.4
atpF_p5	CACCCGCCATTACTTCATCA	CCCATGGGAAGGACTGATTG	50.0	55.0	58.0	57.9	1819-1911 bp	0.991304	86.4