Hotspot

ndhF-rpl32

SSC · 118476-119433 · 958 bp

Back to Quercus

Pi 0.0111

MarkerSeek score 62.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 958
variable_sites: 116
pi: 0.0111
unique_haplotype_count: 84
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCGAAGATTTCTTGTTGCC	TGATATAGAAGTGCGCTTTT	50.0	35.0	58.0	52.7	1052-1164 bp	1.0	52.0
ndhF-rpl32_p2	GCCGAAGATTTCTTGTTGCC	TTGATATAGAAGTGCGCTTT	50.0	35.0	58.0	52.7	1053-1165 bp	1.0	52.0
ndhF-rpl32_p3	GCCGAAGATTTCTTGTTGCC	TTTGATATAGAAGTGCGCTT	50.0	35.0	58.0	52.7	1054-1166 bp	1.0	52.0
ndhF-rpl32_p4	GCCGAAGATTTCTTGTTGCC	TTTTGATATAGAAGTGCGCT	50.0	35.0	58.0	52.7	1055-1167 bp	1.0	52.0
ndhF-rpl32_p5	GCCGAAGATTTCTTGTTGCC	TGATATAGAAGTGCGCTTTTT	50.0	33.3	58.0	53.6	1052-1164 bp	1.0	52.0