Hotspot

petA-psbJ

LSC · 68332-69353 · 1022 bp

Back to Quercus

Pi 0.00965

MarkerSeek score 69.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 96
pi: 0.00965
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1160-1183 bp	1.0	86.7
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1157-1180 bp	1.0	85.6
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1155-1178 bp	1.0	84.2
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	AGGCACTGTAACGGGTATTC	50.0	50.0	58.0	57.3	1219-1242 bp	1.0	83.7
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	GGCACTGTAACGGGTATTCT	50.0	50.0	58.0	57.3	1218-1241 bp	1.0	83.7