Hotspot

ycf4-cemA

LSC · 65813-66443 · 631 bp

Back to Quercus

Pi 0.00688

MarkerSeek score 64.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 52
pi: 0.00688
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGGCGCTATTCCTTTGACTC	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	773-828 bp	1.0	89.1
ycf4-cemA_p2	CTCCACGAGAACTTGAGCAA	GAGATCCACCAGGGCAAAAA	50.0	50.0	57.8	58.1	735-790 bp	1.0	88.1
ycf4-cemA_p3	TGGCGCTATTCCTTTGACTC	GAGAGAGAGATCCACCAGGG	50.0	60.0	58.0	58.3	779-834 bp	1.0	87.3
ycf4-cemA_p4	GACTCCACGAGAACTTGAGC	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.3	58.1	737-792 bp	1.0	86.9
ycf4-cemA_p5	CCATGGCGCTATTCCTTTGA	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.3	58.1	776-831 bp	1.0	86.8