Hotspot

ndhC-trnV-UAC

LSC · 56034-56500 · 467 bp

Back to Quercus

Pi 0.00717

MarkerSeek score 60.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 45
pi: 0.00717
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	566-619 bp	1.0	88.0
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCCGAAAT	TGTTGGGTCTTTGAAACGGT	50.0	45.0	57.9	57.9	627-680 bp	0.991304	87.5
ndhC-trnV-UAC_p3	GAATCGGGGCTAAAACTCCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	571-624 bp	1.0	86.5
ndhC-trnV-UAC_p4	GAATCGGGGCTAAAACTCCC	TGTTGGGTCTTTGAAACGGT	55.0	45.0	58.3	57.9	632-685 bp	0.991304	86.0
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCCGAAAT	TGGGTCTTTGAAACGGTTCA	50.0	45.0	57.9	57.6	624-677 bp	1.0	85.7