Hotspot

trnF-GAA-ndhJ

LSC · 53442-54296 · 855 bp

Back to Quercus

Pi 0.0102

MarkerSeek score 62.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 60
pi: 0.0102
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	925-999 bp	1.0	88.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	961-1035 bp	0.991304	87.8
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	949-1023 bp	1.0	87.3
trnF-GAA-ndhJ_p4	CGGATAATGGTCGGGATAGC	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.7	58.1	973-1047 bp	1.0	85.9
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	970-1044 bp	0.991304	85.8