Hotspot

psaA-ycf3

LSC · 46487-47230 · 744 bp

Back to Quercus

Pi 0.00321

MarkerSeek score 57.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 38
pi: 0.00321
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	903-951 bp	1.0	85.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAACGCTTACTCCCGG	50.0	55.0	57.9	57.9	877-925 bp	1.0	85.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	905-953 bp	1.0	84.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	829-877 bp	0.982609	84.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	828-876 bp	0.982609	84.4