Hotspot

trnS-GCU-trnG-GCC

LSC · 9619-10591 · 973 bp

Back to Quercus

Pi 0.0119
MarkerSeek score 56.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
973
variable_sites
87
pi
0.0119
unique_haplotype_count
62
primer_available
yes
alignment_reliability
0.92
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 952-1067 bp 1.0 81.0
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 951-1066 bp 1.0 81.0
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 952-1067 bp 1.0 81.0
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 951-1066 bp 1.0 81.0
trnS-GCU-trnG-GCC_p5 TAGTCCACTCAGCCATCTCC ACGAATCGCACTTTTACCACT 55.0 42.9 58.2 58.2 939-1054 bp 1.0 79.4