Hotspot

trnK-UUU-rps16

LSC · 4423-5507 · 1085 bp

Back to Quercus

Pi 0.0159

MarkerSeek score 63.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 112
pi: 0.0159
unique_haplotype_count: 91
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAGACTGATCGACGGACTCT	TGTATCGCGCAGGAATCAAA	50.0	45.0	57.9	58.0	2251-2520 bp	1.0	89.2
trnK-UUU-rps16_p2	AGACTGATCGACGGACTCTT	TGTATCGCGCAGGAATCAAA	50.0	45.0	57.9	58.0	2250-2519 bp	1.0	89.2
trnK-UUU-rps16_p3	CCCCCACACTCTAATGGAGA	TGTATCGCGCAGGAATCAAA	55.0	45.0	58.1	58.0	2075-2343 bp	1.0	89.2
trnK-UUU-rps16_p4	AAGTTTTGAACCGATCCGCT	TGTATCGCGCAGGAATCAAA	45.0	45.0	58.1	58.0	1817-2083 bp	0.991304	88.6
trnK-UUU-rps16_p5	GATCGACGGACTCTTTAGCC	TGTATCGCGCAGGAATCAAA	55.0	45.0	57.9	58.0	2245-2514 bp	0.869565	83.3