Hotspot

petA-psbJ

LSC · 66306-67297 · 992 bp

Back to Pyrus

Pi 0.00155
MarkerSeek score 56.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
992
variable_sites
11
pi
0.00155
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGCTCTTCTTGGCATCTGTT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1158-1207 bp 1.0 76.4
petA-psbJ_p2 TTGCTCTTCTTGGCATCTGT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1159-1208 bp 1.0 76.4
petA-psbJ_p3 CGTGTCCAAGGTCTTTTGCT AGCCGGTATTCTTGTGATCG 50.0 50.0 58.7 57.8 1174-1223 bp 1.0 75.4
petA-psbJ_p4 GGTCTTTTGCTCTTCTTGGC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.3 57.8 1165-1214 bp 1.0 75.3
petA-psbJ_p5 TCCATTACGTGTCCAAGGTC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.2 57.8 1181-1230 bp 1.0 74.9