Hotspot

MW450_pgt018-MW450_pgt019

LSC · 49562-50780 · 1219 bp

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Pi 0.00527
MarkerSeek score 58.40
Primer available yes
Label MW450_pgt018-MW450_pgt019

Feature Metadata

label_name
MW450_pgt018-MW450_pgt019
feature_type
igs
region
LSC
length_bp
1219
variable_sites
39
pi
0.00527
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MW450_pgt018-MW450_pgt019_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 1241-1320 bp 1.0 85.3
MW450_pgt018-MW450_pgt019_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 1249-1328 bp 1.0 84.8
MW450_pgt018-MW450_pgt019_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1273-1352 bp 1.0 84.4
MW450_pgt018-MW450_pgt019_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1281-1360 bp 1.0 83.9
MW450_pgt018-MW450_pgt019_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 1255-1334 bp 1.0 83.4