Hotspot

atpH-atpI

LSC · 14720-15895 · 1176 bp

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Pi 0.00202
MarkerSeek score 49.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1176
variable_sites
12
pi
0.00202
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 50.0 50.0 58.0 58.0 1257-1283 bp 1.0 81.1
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1223-1249 bp 1.0 81.1
atpH-atpI_p3 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 50.0 45.0 58.0 57.9 1287-1313 bp 1.0 80.2
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 45.0 45.0 58.0 57.9 1253-1279 bp 1.0 80.2
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1224-1250 bp 1.0 79.9