Hotspot

ycf1

SSC · 121335-126443 · 5109 bp

Back to Pyrrosia

Pi 0.0562

MarkerSeek score 75.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5109
variable_sites: 801
pi: 0.0562
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCGACGAGTAGTTCCGAAC	CAAGATGAACTTCCCCCTCG	55.0	55.0	57.8	58.0	1227 bp	0.2	46.5
ycf1_p2	CTCGACGAGTAGTTCCGAAC	TGTAGCGGCTAGATCAGACA	55.0	50.0	57.8	57.9	1447 bp	0.2	46.3
ycf1_p3	CTCGACGAGTAGTTCCGAAC	CTAACTTCCCCAGCATCGAC	55.0	55.0	57.8	58.1	1506 bp	0.2	46.3
ycf1_p4	CTCGACGAGTAGTTCCGAAC	CCAGCATCGACGGATAAACA	55.0	50.0	57.8	58.1	1497 bp	0.2	46.2
ycf1_p5	ACTCGACGAGTAGTTCCGAA	CAAGATGAACTTCCCCCTCG	50.0	55.0	58.2	58.0	1228 bp	0.2	46.2