Hotspot

rpl2

LSC · 79657-81210 · 1554 bp

Back to Pyrrosia

Pi 0.0501

MarkerSeek score 76.00

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1554
variable_sites: 212
pi: 0.0501
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CTGAACGTGCCATAGCTGAT	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.1	58.0	2097-2138 bp	1.0	89.5
rpl2_p2	CTGAACGTGCCATAGCTGAT	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.1	58.0	2098-2139 bp	1.0	89.5
rpl2_p3	CGAGACCAAGTGACAACCAA	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.1	58.0	2204-2232 bp	0.9	84.9
rpl2_p4	CGAGACCAAGTGACAACCAA	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.1	58.0	2205-2233 bp	0.9	84.9
rpl2_p5	CGAGACCAAGTGACAACCAA	CATCCATGGCTGAACGGTTA	50.0	50.0	58.1	58.0	2242-2270 bp	0.9	84.7