Hotspot

petB

LSC · 70480-71948 · 1469 bp

Back to Pyrrosia

Pi 0.0422

MarkerSeek score 76.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1469
variable_sites: 176
pi: 0.0422
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	ATCCCGCCGACATAACTAGA	GATGATATTCCGCGCTGCTA	50.0	50.0	58.0	58.0	1625 bp	0.2	47.9
petB_p2	TCCCGCCGACATAACTAGAT	GATGATATTCCGCGCTGCTA	50.0	50.0	58.0	58.0	1624 bp	0.2	47.9
petB_p3	ATCCCGCCGACATAACTAGA	GTCACACACTCCCGTAATCC	50.0	55.0	58.0	58.0	1777 bp	0.2	47.9
petB_p4	TCCCGCCGACATAACTAGAT	GTCACACACTCCCGTAATCC	50.0	55.0	58.0	58.0	1776 bp	0.2	47.9
petB_p5	TCCCGCCGACATAACTAGAT	CAACATGGCTTCTGATCCGA	50.0	50.0	58.0	58.0	2268 bp	0.2	47.7