Hotspot

ndhF

SSC · 107450-109669 · 2220 bp

Back to Pyrrosia

Pi 0.0567

MarkerSeek score 79.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 343
pi: 0.0567
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCTTCAAGAAGCGTGGTAG	CTGCATCAGTTAACCACGGA	55.0	50.0	58.3	57.9	2879-2941 bp	1.0	87.7
ndhF_p2	GTAGAATAGCCGCGGGATTC	CTGCATCAGTTAACCACGGA	55.0	50.0	58.3	57.9	2863-2925 bp	1.0	87.6
ndhF_p3	TAGAATAGCCGCGGGATTCT	CTGCATCAGTTAACCACGGA	50.0	50.0	58.4	57.9	2862-2924 bp	1.0	87.0
ndhF_p4	CCCCGACATGTTGTGAGTAG	AGGTGTGACGTGCAAACTTA	55.0	45.0	58.0	57.7	2910 bp	0.2	45.4
ndhF_p5	CCCCGACATGTTGTGAGTAG	TAAGGTGTGACGTGCAAACT	55.0	45.0	58.0	57.7	2912 bp	0.2	45.4