Hotspot

rpl32-trnP-GGG

SSC · 110776-111378 · 603 bp

Back to Pyrrosia

Pi 0.11
MarkerSeek score 72.60
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
603
variable_sites
182
pi
0.11
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 CCTTACGTTCCTGCTCCTTC CGCAAAAGCCCCTCTCTAAT 55.0 50.0 58.0 58.0 1120-1152 bp 0.2 47.6
rpl32-trnP-GGG_p2 CTTCCTTACGTTCCTGCTCC CGCAAAAGCCCCTCTCTAAT 55.0 50.0 58.0 58.0 1123-1155 bp 0.2 47.6
rpl32-trnP-GGG_p3 CCTTACGTTCCTGCTCCTTC GCTTGCTGAAACAGTTGCAA 55.0 45.0 58.0 58.1 1098-1130 bp 0.2 47.3
rpl32-trnP-GGG_p4 CTTCCTTACGTTCCTGCTCC GCTTGCTGAAACAGTTGCAA 55.0 45.0 58.0 58.1 1101-1133 bp 0.2 47.3
rpl32-trnP-GGG_p5 CCTTACGTTCCTGCTCCTTC TGTTTCACCATAGCGGCTTT 55.0 45.0 58.0 58.1 1243-1275 bp 0.2 47.1