Hotspot

clpP-psbB

LSC · 67430-67899 · 470 bp

Back to Pyrrosia

Pi 0.0553

MarkerSeek score 75.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 73
pi: 0.0553
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CCTTTGGAACACCAATTGGC	GATACCAAGGCAAACCCGT	50.0	52.6	57.8	57.7	498-511 bp	1.0	80.6
clpP-psbB_p2	CCTTTGGAACACCAATTGGC	CGATACCAAGGCAAACCCG	50.0	57.9	57.8	58.9	499-512 bp	1.0	75.4
clpP-psbB_p3	CTTTGGAACACCAATTGGCA	GATACCAAGGCAAACCCGT	45.0	52.6	57.1	57.7	497-510 bp	1.0	74.7
clpP-psbB_p4	GCACCTTTGGAACACCAATT	GATACCAAGGCAAACCCGT	45.0	52.6	57.1	57.7	501-514 bp	1.0	74.7
clpP-psbB_p5	GGCACCTTTGGAACACCAAT	GATACCAAGGCAAACCCGT	50.0	52.6	59.0	57.7	502-515 bp	1.0	74.3