Hotspot

trnL-UAA-trnF-GAA

LSC · 44628-44878 · 251 bp

Back to Pyrrosia

Pi 0.0774

MarkerSeek score 76.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 56
pi: 0.0774
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TTCGAATCCCTCTATCCCCA	GTGACACGGGGATTTTCAGT	50.0	50.0	57.2	58.1	321-341 bp	1.0	82.1
trnL-UAA-trnF-GAA_p2	TTCGAATCCCTCTATCCCCA	TGACACGGGGATTTTCAGTC	50.0	50.0	57.2	57.8	320-340 bp	1.0	81.5
trnL-UAA-trnF-GAA_p3	TTCGAATCCCTCTATCCCCA	GGATTTTCAGTCCCCTGCTC	50.0	55.0	57.2	58.2	312-332 bp	1.0	81.0
trnL-UAA-trnF-GAA_p4	AAATCCGTTGGTTTCACGGG	GTGACACGGGGATTTTCAGT	50.0	50.0	59.0	58.1	351-371 bp	1.0	79.9
trnL-UAA-trnF-GAA_p5	TTCGAATCCCTCTATCCCCA	ATTTGAACTGGTGACACGGG	50.0	50.0	57.2	58.4	331-351 bp	1.0	79.7