Hotspot

trnC-GCA-trnG-GCC

LSC · 28450-28954 · 505 bp

Back to Pyrrosia

Pi 0.0883
MarkerSeek score 75.70
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
LSC
length_bp
505
variable_sites
124
pi
0.0883
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 TTACCGCTTGACTATGTCGC GTTCGATTCCCGCTATCCG 50.0 57.9 58.1 58.2 494-547 bp 1.0 81.8
trnC-GCA-trnG-GCC_p2 TTACCGCTTGACTATGTCGC GGGTTCGATTCCCGCTATC 50.0 57.9 58.1 57.8 496-549 bp 1.0 81.6
trnC-GCA-trnG-GCC_p3 TTACCGCTTGACTATGTCGC GGTTCGATTCCCGCTATCC 50.0 57.9 58.1 57.8 495-548 bp 1.0 81.6
trnC-GCA-trnG-GCC_p4 TTACCGCTTGACTATGTCGC CTTGCCAAGGAGATGACGC 50.0 57.9 58.1 58.9 515-568 bp 1.0 76.1
trnC-GCA-trnG-GCC_p5 TTACCGCTTGACTATGTCGC GATGACGCGGGTTCGATTC 50.0 57.9 58.1 59.1 504-557 bp 1.0 74.7