Hotspot

petN-trnC-GCA

LSC · 27905-28377 · 473 bp

Back to Pyrrosia

Pi 0.0581

MarkerSeek score 75.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 71
pi: 0.0581
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TGTGGAAATCACCGTCAAGG	CCAGTTCGAATCTGGGTGTC	50.0	55.0	58.1	58.3	517-545 bp	1.0	88.9
petN-trnC-GCA_p2	GCTTCGTCCCCAAACTACAA	CCAGTTCGAATCTGGGTGTC	50.0	55.0	58.1	58.3	550-578 bp	1.0	88.9
petN-trnC-GCA_p3	TGTGGAAATCACCGTCAAGG	CAGTTCGAATCTGGGTGTCG	50.0	55.0	58.1	58.6	516-544 bp	1.0	85.9
petN-trnC-GCA_p4	GCTTCGTCCCCAAACTACAA	CAGTTCGAATCTGGGTGTCG	50.0	55.0	58.1	58.6	549-577 bp	1.0	85.9
petN-trnC-GCA_p5	CGTCCCCAAACTACAAGTGA	CCAGTTCGAATCTGGGTGTC	50.0	55.0	57.5	58.3	546-574 bp	1.0	85.5