Hotspot

atpH-atpI

LSC · 12280-13036 · 757 bp

Back to Pyrrosia

Pi 0.067

MarkerSeek score 76.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 138
pi: 0.067
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCCTCCTGGAAAGTTTTGC	GAATACCGTTCCTGGGTTCC	50.0	55.0	58.0	58.0	1795-1827 bp	1.0	90.0
atpH-atpI_p2	TTCTGGCTGTCTCGCAATAC	GAATACCGTTCCTGGGTTCC	50.0	55.0	58.0	58.0	1332-1366 bp	1.0	90.0
atpH-atpI_p3	TCCCTCCTGGAAAGTTTTGC	AGGGATTTAACGAGGACCCA	50.0	50.0	58.0	58.0	1828-1860 bp	1.0	89.9
atpH-atpI_p4	TCCCTCCTGGAAAGTTTTGC	GGTAGTAGCTGTCCTCGTCT	50.0	55.0	58.0	58.0	1466-1498 bp	0.9	86.4
atpH-atpI_p5	TCCCTCCTGGAAAGTTTTGC	TGGGGGAAGAAGAATACCGT	50.0	50.0	58.0	58.0	1818-1838 bp	0.4	63.6