Hotspot

matK-chlB

LSC · 1726-3507 · 1782 bp

Back to Pyrrosia

Pi 0.0855

MarkerSeek score 79.80

Primer available yes

Label matK-chlB

Feature Metadata

label_name: matK-chlB
feature_type: igs
region: LSC
length_bp: 1782
variable_sites: 434
pi: 0.0855
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-chlB_p1	TTTCTGTTTCCGAAGCCGAA	TTGGAGATACGATAGCCCGT	45.0	50.0	58.0	58.0	2782-2843 bp	1.0	91.2
matK-chlB_p2	TTCTGTTTCCGAAGCCGAAA	TTGGAGATACGATAGCCCGT	45.0	50.0	58.0	58.0	2781-2842 bp	0.9	87.1
matK-chlB_p3	TTTTCTGTTTCCGAAGCCGA	TTGGAGATACGATAGCCCGT	45.0	50.0	58.0	58.0	2783-2844 bp	0.6	74.8
matK-chlB_p4	TTCTGTTTCCGAAGCCGAAA	ACACGACACTAAGGGGGTAA	45.0	50.0	58.0	58.0	2601 bp	0.2	47.5
matK-chlB_p5	TTTCTGTTTCCGAAGCCGAA	ACACGACACTAAGGGGGTAA	45.0	50.0	58.0	58.0	2602 bp	0.2	47.5