Hotspot

ndhA

SSC · 122327-124521 · 2195 bp

Back to Pyrenaria

Pi 0.00178

MarkerSeek score 52.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2195
variable_sites: 13
pi: 0.00178
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	CCGCCAGGTTTTGTCAATTT	47.6	45.0	58.1	57.5	2213-2429 bp	1.0	71.7
ndhA_p2	TGAACCCAGTTACCATAGGGA	CCGCCAGGTTTTGTCAATTT	47.6	45.0	57.8	57.5	2216-2432 bp	1.0	70.7
ndhA_p3	CCCAGTTACCATAGGGAACA	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2212-2428 bp	1.0	66.0
ndhA_p4	TGAACCCAGTTACCATAGGG	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2216-2432 bp	1.0	66.0
ndhA_p5	ACCCAGTTACCATAGGGAAC	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2213-2429 bp	1.0	66.0