Hotspot

trnT-UGU-trnL-UAA

LSC · 48686-49692 · 1007 bp

Back to Pyrenaria

Pi 0.00329

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 11
pi: 0.00329
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1058-1089 bp	1.0	82.4
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1066-1097 bp	1.0	81.9
trnT-UGU-trnL-UAA_p3	AAATGCGATGCTCTAACCCC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.3	58.0	1030-1061 bp	1.0	81.9
trnT-UGU-trnL-UAA_p4	AAATGCGATGCTCTAACCCC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.3	58.1	1038-1069 bp	1.0	81.3
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1072-1103 bp	1.0	80.5