Hotspot

rps16-trnQ-UUG

LSC · 6189-7810 · 1622 bp

Back to Pyrenaria

Pi 0.0043

MarkerSeek score 56.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1622
variable_sites: 22
pi: 0.0043
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1704-1712 bp	1.0	80.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1687-1695 bp	1.0	79.9
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	TAGAAACGAAAATGGGGCGT	50.0	45.0	57.9	57.8	1764-1772 bp	1.0	79.4
rps16-trnQ-UUG_p4	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1705-1713 bp	1.0	79.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAGAAACGAAAATGGGGCGT	45.0	45.0	58.1	57.8	1747-1755 bp	1.0	78.9