Hotspot

trnH-GUG-psbA

LSC · 77-458 · 382 bp

Back to Pyrenaria

Pi 0.00838

MarkerSeek score 60.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 9
pi: 0.00838
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	485-508 bp	1.0	81.7
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	486-509 bp	1.0	81.4
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	486-509 bp	1.0	81.2
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	487-510 bp	1.0	81.0
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	478-501 bp	1.0	80.1