Hotspot

ycf1

Genome · 131541-137180 · 5640 bp

Back to Pyracantha

Pi 0.000355

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5640
variable_sites: 2
pi: 0.000355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACGATTCCTGAATCATCCGA	TGAACCGATTTTAAAAGAACTCA	47.6	30.4	57.6	54.8	282 bp	1.0	40.3
ycf1_p2	ACGATTCCTGAATCATCCGA	TGAACCGATTTTAAAAGAACTCA	45.0	30.4	56.4	54.8	281 bp	1.0	40.3
ycf1_p3	GGACGATTCCTGAATCATCCG	TGAACCGATTTTAAAAGAACTCA	52.4	30.4	58.6	54.8	283 bp	1.0	40.3
ycf1_p4	GACGATTCCTGAATCATCCGA	TGAACCGATTTTAAAAGAACTCAA	47.6	29.2	57.6	55.5	282 bp	1.0	40.3
ycf1_p5	GACGATTCCTGAATCATCCGA	TTGAACCGATTTTAAAAGAACTCA	47.6	29.2	57.6	55.5	283 bp	1.0	40.3