Hotspot

rpl16

Genome · 87430-88819 · 1390 bp

Back to Pyracantha

Pi 0

MarkerSeek score 59.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1390
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCCAAAACCCCTTTCGTTTG	TGGCAAACTCTTTCATTCTT	50.0	35.0	57.8	53.3	1515-1519 bp	1.0	40.5
rpl16_p2	TCATCCCCAAAACCCCTTTC	TGGCAAACTCTTTCATTCTT	50.0	35.0	57.7	53.3	1520-1524 bp	1.0	40.2
rpl16_p3	CCCAAAACCCCTTTCGTTTG	TGGCAAACTCTTTCATTCTTT	50.0	33.3	57.8	54.2	1515-1519 bp	1.0	40.2
rpl16_p4	TCATCCCCAAAACCCCTTTC	TGGCAAACTCTTTCATTCTTT	50.0	33.3	57.7	54.2	1520-1524 bp	1.0	40.2
rpl16_p5	CCCAAAACCCCTTTCGTTTG	ATGGCAAACTCTTTCATTCT	50.0	35.0	57.8	53.0	1516-1520 bp	1.0	40.2