Hotspot

ndhF-rpl32

Genome · 119028-120153 · 1126 bp

Back to Pyracantha

Pi 0.00267

MarkerSeek score 59.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1126
variable_sites: 3
pi: 0.00267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	1322-1325 bp	1.0	70.6
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	40.0	45.0	57.0	57.8	1320-1323 bp	1.0	70.3
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	40.0	55.0	57.0	58.3	1309-1312 bp	1.0	69.3
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	40.0	45.0	57.0	57.6	1319-1322 bp	1.0	68.4
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	1323-1326 bp	1.0	68.4