Hotspot

petA-psbJ

Genome · 68545-69532 · 988 bp

Back to Pyracantha

Pi 0

MarkerSeek score 58.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 988
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1157-1159 bp	1.0	73.7
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1158-1160 bp	1.0	73.7
petA-psbJ_p3	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1164-1166 bp	1.0	72.6
petA-psbJ_p4	TGCTCTTCTTGGCATCTGTT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1105-1107 bp	1.0	71.6
petA-psbJ_p5	TTGCTCTTCTTGGCATCTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1106-1108 bp	1.0	71.6