Hotspot

rpoB-trnC-GCA

Genome · 30147-31365 · 1219 bp

Back to Pyracantha

Pi 0.00082

MarkerSeek score 59.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1219
variable_sites: 1
pi: 0.00082
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1374 bp	1.0	78.4
rpoB-trnC-GCA_p2	TAAGCCCTGCTCAATGAACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1356 bp	1.0	76.2
rpoB-trnC-GCA_p3	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1308 bp	1.0	75.8
rpoB-trnC-GCA_p4	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1380 bp	1.0	75.6
rpoB-trnC-GCA_p5	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.5	58.1	1384 bp	1.0	75.0