Hotspot

trnR-UCU-atpA

Genome · 10234-13078 · 2845 bp

Back to Pyracantha

Pi 0.00242

MarkerSeek score 55.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 2845
variable_sites: 5
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGACTTGAATAAGAGACGCTCT	ACAATCTCAATCATCCCCGC	40.9	50.0	56.9	58.0	1030-1098 bp	1.0	59.7
trnR-UCU-atpA_p2	TGACTTGAATAAGAGACGCTCT	TTCGGTTTCCAGGGTAGGAT	40.9	50.0	56.9	58.0	1210-1278 bp	1.0	59.0
trnR-UCU-atpA_p3	TGACTTGAATAAGAGACGCTCT	TCAGACCGGCCATTAATGTG	40.9	50.0	56.9	58.0	1235-1303 bp	1.0	58.9
trnR-UCU-atpA_p4	TGACTTGAATAAGAGACGCTCT	CCGCAGCTCAAATTAAAGCC	40.9	50.0	56.9	58.1	1190-1258 bp	1.0	58.6
trnR-UCU-atpA_p5	TGACTTGAATAAGAGACGCTCT	CGAAGCTTATCCGGGAGATG	40.9	55.0	56.9	57.9	1444-1512 bp	1.0	58.1