Hotspot

trnQ-UUG-psbK

Genome · 7302-7765 · 464 bp

Back to Pyracantha

Pi 0.00216

MarkerSeek score 57.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: Genome
length_bp: 464
variable_sites: 1
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CGTGGGTCAGGTATGTTCTG	GGCAATTTGGCGACGAAAAA	55.0	45.0	58.0	58.5	633 bp	1.0	76.0
trnQ-UUG-psbK_p2	TCGTGGGTCAGGTATGTTCT	GGCAATTTGGCGACGAAAAA	50.0	45.0	58.1	58.5	634 bp	1.0	75.5
trnQ-UUG-psbK_p3	TGATCGTGGGTCAGGTATGT	GGCAATTTGGCGACGAAAAA	50.0	45.0	58.1	58.5	637 bp	1.0	75.0
trnQ-UUG-psbK_p4	GTCAGGTATGTTCTGGGACG	GGCAATTTGGCGACGAAAAA	55.0	45.0	57.7	58.5	628 bp	1.0	73.7
trnQ-UUG-psbK_p5	GTGGGTCAGGTATGTTCTGG	GGCAATTTGGCGACGAAAAA	55.0	45.0	57.6	58.5	632 bp	1.0	72.8