Hotspot

trnH-GUG-psbA

Genome · 281-541 · 261 bp

Back to Pyracantha

Pi 0

MarkerSeek score 25.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 261
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	436 bp	1.0	79.1
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	386 bp	1.0	78.9
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	389 bp	1.0	78.8
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	389 bp	1.0	78.7
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	388 bp	1.0	78.7