Hotspot

ndhA

SSC · 124363-126512 · 2150 bp

Pi 0.00312

MarkerSeek score 52.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2150
variable_sites: 20
pi: 0.00312
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACGTTTCTTTCTCTTGTTTGA	TCCGCCCGGTTTTATCAATT	34.8	45.0	56.9	57.9	2334-2341 bp	1.0	46.9
ndhA_p2	TGACGTTTCTTTCTCTTGTTTGA	TGGGAGAAGTTGATCGGTGA	34.8	50.0	56.9	58.4	2236-2243 bp	1.0	45.1
ndhA_p3	TTGACGTTTCTTTCTCTTGTTTGA	TCCGCCCGGTTTTATCAATT	33.3	45.0	57.5	57.9	2335-2342 bp	1.0	44.0
ndhA_p4	TGACGTTTCTTTCTCTTGTTTG	TCCGCCCGGTTTTATCAATT	36.4	45.0	55.5	57.9	2334-2341 bp	1.0	43.6
ndhA_p5	TGACGTTTCTTTCTCTTGTTTGA	GTCCGCCCGGTTTTATCAAT	34.8	50.0	56.9	58.6	2335-2342 bp	1.0	43.0