Hotspot

rps15-ycf1

SSC · 128067-128459 · 393 bp

Pi 0.00741

MarkerSeek score 50.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 393
variable_sites: 8
pi: 0.00741
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAAATGTGAAGTAAGTCTCCGT	TGGCAGTCGTTTCAGTATGT	40.9	45.0	56.4	57.2	571-574 bp	1.0	45.6
rps15-ycf1_p2	AGTCTCCGTATCTTATTGGTGA	TGGCAGTCGTTTCAGTATGT	40.9	45.0	56.1	57.2	558-561 bp	1.0	43.3
rps15-ycf1_p3	TGTGAAGTAAGTCTCCGTATCT	TGGCAGTCGTTTCAGTATGT	40.9	45.0	56.1	57.2	567-570 bp	1.0	43.0
rps15-ycf1_p4	AATGTGAAGTAAGTCTCCGT	TGGCAGTCGTTTCAGTATGT	40.0	45.0	54.0	57.2	569-572 bp	1.0	42.7
rps15-ycf1_p5	CAAATGTGAAGTAAGTCTCCGT	GGCAGTCGTTTCAGTATGTCA	40.9	47.6	56.4	58.3	570-573 bp	1.0	41.8