Hotspot

rpl32-trnL-UAG

SSC · 117865-118627 · 763 bp

Back to Puya

Pi 0.00894
MarkerSeek score 49.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
763
variable_sites
17
pi
0.00894
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CACCGGGCATTCAAAAAGTT TCGAACCGAGATGCTTTAGC 45.0 50.0 57.5 58.1 885-888 bp 1.0 76.9
rpl32-trnL-UAG_p2 CACCGGGCATTCAAAAAGTT TTTTACAAGATGCCGCCACT 45.0 45.0 57.5 58.1 910-913 bp 1.0 76.7
rpl32-trnL-UAG_p3 CACCGGGCATTCAAAAAGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.5 57.9 859-862 bp 1.0 76.6
rpl32-trnL-UAG_p4 TTCCACCGGGCATTCAAAAA TCGAACCGAGATGCTTTAGC 45.0 50.0 58.6 58.1 888-891 bp 1.0 76.4
rpl32-trnL-UAG_p5 TTTCCACCGGGCATTCAAAA TCGAACCGAGATGCTTTAGC 45.0 50.0 58.6 58.1 889-892 bp 1.0 76.4